Quick Details

  • CAS No.: 369-07-3
  • Other Names: ONPG
  • MF: C12H15NO8
  • EINECS No.: 206-716-1
  • Place of Origin: Karnataka, India
  • Type: Pharmaceutical Intermediates
  • Purity: 99%
  • Brand Name: JASMA
  • Model Number: JASMA04
  • Application: Synthetic Colors
  • Appearance: White

Packaging & Delivery

Packaging Details: Standard
Delivery Detail: 1-2 weeks


ortho-Nitrophenyl-aa-galactoside (ONPG) is a colorimetric and spectrophotometric substrate for detection of beta-galactosidase.

Ortho-Nitrophenyl-β-Galactoside (ONPG) is a colorimetric and spectrophotometric substrate for detection of beta-galactosidase activity.

  • This compound is normally colorless. However if β-galactosidase is present, it hydrolyzes the ONPG molecule into galactose and ortho-nitrophenol.
  • The latter compound has a yellow color that can be used to check for enzyme activity by means of a colorimetric assay (at 420 nm wavelength). β-galactosidase is required for lactose utilization, so the intensity of the color produced can be used as a measure of the enzymatic rate.
  • Though ONPG mimics lactose and is hydrolyzed by β-galactosidase, it is unable to act as an inducer for the lac operon. Without another lactose analog that can act as an inducer, such as IPTG, β-galactosidase will not be transcribed and ONPG will not be hydrolyzed.
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